# Make.shared error when used after phylotype

**URL:** <https://forum.mothur.org/t/make-shared-error-when-used-after-phylotype/22219>\
**Category:** mothur bugs\
**Created:** [October 4, 2024, 9:30pm UTC](https://forum.mothur.org/t/make-shared-error-when-used-after-phylotype/22219 "2024-10-04T21:30:37Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![nohayoussef](https://avatars.discourse-cdn.com/v4/letter/n/ccd318/32.png) [@nohayoussef](https://forum.mothur.org/u/nohayoussef)\
**Post date:** [October 4, 2024, 9:30pm UTC](https://forum.mothur.org/t/make-shared-error-when-used-after-phylotype/22219/1 "2024-10-04T21:30:37Z")

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Hi all,  
I am working with LSU fungal sequences. I use Blast against an in-house database to classify the sequences and then create a .taxonomy file from the Blast output (long story but classify.seqs against my database puts the majority of my sequences in the unclassifed bin so I had to resort to Blast for classification and then come back to Mothur for making the shared file). Anywho, so I have that taxonomy file based on my Blast results which I then use in the phylotype command to create a .tx.list. All good so far. When I then use the .tx.list and the .count\_table in the make.shared command, I get an error that there are sequences in my list that are not in the count. Well, there are not. I checked and re-checked and the number and the names of the sequences in the list and count are a compete match. I am using v.1.48.

I would really appreciate any and all help and thank you so much in advance.  
P.S. I advocate for Mothur all the time btw.

Thanks  
Noha

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**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [October 14, 2024, 9:30pm UTC](https://forum.mothur.org/t/make-shared-error-when-used-after-phylotype/22219/2 "2024-10-14T21:30:44Z")

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [October 16, 2024, 8:56pm UTC](https://forum.mothur.org/t/make-shared-error-when-used-after-phylotype/22219/3 "2024-10-16T20:56:06Z")

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If you could send us the files ([mothur.bugs@gmail.com](mailto:mothur.bugs@gmail.com)) and reference this post we could take a look  
Pat