# make.contigs

**URL:** <https://forum.mothur.org/t/make-contigs/2245>\
**Category:** Commands in mothur\
**Created:** [February 10, 2015, 9:54am UTC](https://forum.mothur.org/t/make-contigs/2245 "2015-02-10T09:54:58Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Egansbay](https://avatars.discourse-cdn.com/v4/letter/e/ad7895/32.png) [@Egansbay](https://forum.mothur.org/u/Egansbay)\
**Post date:** [February 10, 2015, 9:54am UTC](https://forum.mothur.org/t/make-contigs/2245/1 "2015-02-10T09:54:58Z")

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Deal all

I am trying to run analysis of one single set of MiSeq 16S data on Mothur. I am using Windows 8 (64 bit exec verison of Mothur downloaded)

I have all the files in the same folder, ready to go.

I rename my forward and reverse read files to ng1.fastq and ng2.fastq

Command I input is make.contigs(ffastq=ng1.fastq, rfastq=ng2.fastq) but no luck, Mothur can’t open the files?

Can anyone advise me on this issue…soul destroying!

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [February 10, 2015, 2:16pm UTC](https://forum.mothur.org/t/make-contigs/2245/2 "2015-02-10T14:16:40Z")

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This may be helpful… [http://www.mothur.org/wiki/Frequently\_asked\_questions#Mothur\_can.27t\_find\_my\_input\_files](http://www.mothur.org/wiki/Frequently_asked_questions#Mothur_can.27t_find_my_input_files)