Make contigs with NextFLEX 18S ITS kit

Commands in mothur
1

Hi

I’ve used NextFLEX 16s in mothur before, but has anybody tried the 18s ITS kit.

Not sure I understand correctly, does forward and reverse reads need to overlap when making contigs?

Cheers
Erik

2

They must overlap:

http://blog.mothur.org/2014/09/11/Why-such-a-large-distance-matrix%3F/