# make.contigs--Segmentation fault: 11

**URL:** <https://forum.mothur.org/t/make-contigs-segmentation-fault-11/20124>\
**Category:** mothur bugs\
**Created:** [September 27, 2019, 4:39pm UTC](https://forum.mothur.org/t/make-contigs-segmentation-fault-11/20124 "2019-09-27T16:39:53Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![micah\_rain](https://avatars.discourse-cdn.com/v4/letter/m/f4b2a3/32.png) [@micah\_rain](https://forum.mothur.org/u/micah_rain)\
**Post date:** [September 27, 2019, 4:39pm UTC](https://forum.mothur.org/t/make-contigs-segmentation-fault-11/20124/1 "2019-09-27T16:39:53Z")

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Hi there,

I am getting the following error when trying to run the make.contigs command. The previous files (1-17) aren’t having any issues. Not sure what to do… --Micah

mothur \> make.contigs(file=hm.paired.files)

> > > > > Processing file pair 7\_S172\_Sept\_18\_L001\_R1\_001.fastq - 7\_S182\_Sept\_18\_L001\_R1\_001.fastq (files 18 of 18) \<\<\<\<\<  
> > > > > Making contigs…  
> > > > > M01479\_272\_000000000-C97K2\_1\_1102\_16333\_1373 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_1104\_13608\_19089 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_1107\_3418\_10751 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_1112\_13646\_26704 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_2102\_10003\_16258 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_2103\_6667\_25980 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_2107\_12854\_11036 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > M01479\_272\_000000000-C97K2\_1\_2112\_6123\_20237 is in your forward fastq file and not in your reverse file, please remove it using the remove.seqs command before proceeding.  
> > > > > Segmentation fault: 11

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [October 2, 2019, 3:35pm UTC](https://forum.mothur.org/t/make-contigs-segmentation-fault-11/20124/2 "2019-10-02T15:35:58Z")

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“Processing file pair 7\_S172\_Sept\_18\_L001\_R1\_001.fastq - 7\_S182\_Sept\_18\_L001\_R1\_001.fastq”

These both appear to be forward files from different samples based on the file names. Could this be a typo in the file file?

7\_S172\_Sept\_18\_L001\_R1\_001.fastq  
7\_S182\_Sept\_18\_L001\_R1\_001.fastq

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**Author:** ![micah\_rain](https://avatars.discourse-cdn.com/v4/letter/m/f4b2a3/32.png) [@micah\_rain](https://forum.mothur.org/u/micah_rain)\
**Post date:** [October 9, 2019, 4:27pm UTC](https://forum.mothur.org/t/make-contigs-segmentation-fault-11/20124/3 "2019-10-09T16:27:06Z")

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That could very well be… will check it out–thank you!

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**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [October 19, 2019, 4:27pm UTC](https://forum.mothur.org/t/make-contigs-segmentation-fault-11/20124/4 "2019-10-19T16:27:08Z")

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