# Make.contigs doesn't create stability.contigs.count\_table **URL:** \ **Category:** mothur bugs\ **Created:** [August 25, 2022, 2:32pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545 "2022-08-25T14:32:22Z")\ **Posts on this page:** 8\ **Page:** 1
**Author:** ![Sithara85](https://avatars.discourse-cdn.com/v4/letter/s/bbe5ce/32.png) [@Sithara85](https://forum.mothur.org/u/Sithara85)\ **Post date:** [August 25, 2022, 2:32pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/1 "2022-08-25T14:32:22Z")
Hi, I couldn’t find stability.contigs.count\_table to run commands like summary.seqs(fasta=stability.trim.contigs.fasta, count=stability.contigs.count\_table). Please see attached the files make.contigs created. I didn’t have this problem earlier when I used Mothur. I tried various versions but none output count\_table from make.contigs. Is it Ok to proceed? ![image](https://canada1.discourse-cdn.com/flex036/uploads/mothur/original/1X/935b0acc01f9b8b865bbf239fca09c473fc73f7f.png) ---
**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [August 25, 2022, 4:18pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/2 "2022-08-25T16:18:14Z")
Hi, I suspect you have an older version of mothur. If you open that mothur logfile that’s at the top you’ll find the version number towards the top. This was a change that was made in recent versions of mothur. Pat ---
**Author:** ![Sithara85](https://avatars.discourse-cdn.com/v4/letter/s/bbe5ce/32.png) [@Sithara85](https://forum.mothur.org/u/Sithara85)\ **Post date:** [August 25, 2022, 4:33pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/3 "2022-08-25T16:33:01Z")
Yeah, I thought so…but I used mothur/1.45.3 still the same result. I run Mothur from our supercomputer institute, and last year it worked fine. ---
**Author:** ![Sithara85](https://avatars.discourse-cdn.com/v4/letter/s/bbe5ce/32.png) [@Sithara85](https://forum.mothur.org/u/Sithara85)\ **Post date:** [August 26, 2022, 8:15pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/4 "2022-08-26T20:15:06Z")
Hi Pat, Thank you for your response! We have updated the software to 1.48.0 and now I have the make.contigs function create all the output files as expected. Output File Names: stability.trim.contigs.fasta stability.scrap.contigs.fasta stability.contigs\_report stability.contigs.count\_table ---
**Author:** ![Sithara85](https://avatars.discourse-cdn.com/v4/letter/s/bbe5ce/32.png) [@Sithara85](https://forum.mothur.org/u/Sithara85)\ **Post date:** [August 30, 2022, 1:53am UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/5 "2022-08-30T01:53:13Z")
Hi Pat, Hopefully one last question, I am running 12 samples to set up Mothur to run some clinical experiment samples. I followed the Miseq SOP. Could you tell me why the dist.seqs(fasta=final.fasta, cutoff=0.03) is taking ever to finish?Is it expected or am I doing something stupid? I am running it on a supercomputer using 900 GB of memory. Thank you, Sithara ---
**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [August 30, 2022, 4:32pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/6 "2022-08-30T16:32:56Z")
I suspect you have a ton of sequences. Are you sequencing the V4 region or another region? Regardless, you should probably checkout using `cluster.split` instead. You can see how to use this in the mothur MiSeq SOP. Pat ---
**Author:** ![Sithara85](https://avatars.discourse-cdn.com/v4/letter/s/bbe5ce/32.png) [@Sithara85](https://forum.mothur.org/u/Sithara85)\ **Post date:** [August 30, 2022, 4:46pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/7 "2022-08-30T16:46:28Z")
Ok Thank you! I think I have about 11 million seqs. I will try cluster.split and see how it works. summary.seqs(fasta=current, count=current) ./stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.vsearch.pick.count\_table ./stability.trim.contigs.good.unique.good.filter.unique.precluster.denovo.vsearch.pick.fasta Using 32 processors. ``` Start End NBases Ambigs Polymer NumSeqs ``` Minimum: 1 340 173 0 3 1 2.5%-tile: 1 340 180 0 4 283720 25%-tile: 1 340 180 0 6 2837191 Median: 1 340 180 0 6 5674382 75%-tile: 1 340 180 0 6 8511572 97.5%-tile: 1 340 180 4 6 11065043 Maximum: 2 340 185 27 8 11348762 Mean: 1 340 179 0 5 # of unique seqs: 412530 total # of seqs: 11348762 ---
**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\ **Post date:** [September 9, 2022, 4:47pm UTC](https://forum.mothur.org/t/make-contigs-doesnt-create-stability-contigs-count-table/21545/8 "2022-09-09T16:47:21Z")
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