I used make.contigs to merger read.1 and read.2. I also provided barcode oligos to separate samples. Both the forward and reverse barcode included 8 nucleotides. In the scarp fasta file, I got the following sequence. May I ask the meaning of “rbdiffs=1001(noMatch)”. Does it mean the reverse barcode absent in the sequence?
M00278_49_000000000-CPBKN_1_1108_23033_3567 | b(b) ee=6.87543 fbdiffs=3(noMatch), rbdiffs=1001(noMatch) fpdiffs=0(match), rpdiffs=0(match)
The other question is the meaning of “fbdiffs=0(noMatch), rbdiffs=3(noMatch)”.
Does it mean that forward barcode is matched but there were 3 mismatches in the reverse barcode? Therefore, this sequence was discarded.
Thank you for your kindly reply