# Mac executable version

**URL:** <https://forum.mothur.org/t/mac-executable-version/644>\
**Category:** mothur bugs\
**Created:** [August 29, 2011, 11:52pm UTC](https://forum.mothur.org/t/mac-executable-version/644 "2011-08-29T23:52:50Z")\
**Posts on this page:** 7\
**Page:** 1

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**Author:** ![transcription](https://avatars.discourse-cdn.com/v4/letter/t/7bcc69/32.png) [@transcription](https://forum.mothur.org/u/transcription)\
**Post date:** [August 29, 2011, 11:52pm UTC](https://forum.mothur.org/t/mac-executable-version/644/1 "2011-08-29T23:52:50Z")

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I’ve wanted to use Mothur for my 454 sequencing data. Downloaded 64-bit mac executable version but mother doesn’t want to run any commands even summar.seqs.

What might be the problem?

Thanks,

Fey

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [August 30, 2011, 12:45pm UTC](https://forum.mothur.org/t/mac-executable-version/644/2 "2011-08-30T12:45:52Z")

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Could you post the error message you are receiving?

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**Author:** ![transcription](https://avatars.discourse-cdn.com/v4/letter/t/7bcc69/32.png) [@transcription](https://forum.mothur.org/u/transcription)\
**Post date:** [August 31, 2011, 11:23pm UTC](https://forum.mothur.org/t/mac-executable-version/644/3 "2011-08-31T23:23:02Z")

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It is

Unable to open holme.fas  
[ERROR]: did not complete summary.seqs.

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [September 1, 2011, 10:57am UTC](https://forum.mothur.org/t/mac-executable-version/644/4 "2011-09-01T10:57:45Z")

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The most common cause of mothur not finding a file is because you double clicked on the mothur executable to run mothur. This will open a terminal or command prompt window in your home directory. Mothur will then look for the input files in the home directory instead of in the directory where mothur’s executable is located. If this is the cause, you either put the input files in your home directory, give complete file names, or open a terminal or command prompt window, cd into the directory where the mothur executable is located and run mothur by using “./mothur” for mac or “mothur” for windows.

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**Author:** ![transcription](https://avatars.discourse-cdn.com/v4/letter/t/7bcc69/32.png) [@transcription](https://forum.mothur.org/u/transcription)\
**Post date:** [September 2, 2011, 5:58am UTC](https://forum.mothur.org/t/mac-executable-version/644/5 "2011-09-02T05:58:40Z")

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It works! Thanks, you are really helpful 🙂

Fey

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**Author:** ![transcription](https://avatars.discourse-cdn.com/v4/letter/t/7bcc69/32.png) [@transcription](https://forum.mothur.org/u/transcription)\
**Post date:** [September 4, 2011, 5:01am UTC](https://forum.mothur.org/t/mac-executable-version/644/6 "2011-09-04T05:01:10Z")

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Hi,

I wanted to run align.seqs command (using silva template), but getting this error message;

Unable to open silva.bacteria.fasta  
[ERROR]: did not complete align.seqs.

All the files related to mothur are in the home directory…

Thanks in advance,

Fey

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [October 4, 2011, 12:24pm UTC](https://forum.mothur.org/t/mac-executable-version/644/7 "2011-10-04T12:24:47Z")

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Sorry this looks like a very old message that we missed - are you sure that silva.bacteria.fasta is in the same folder as your data and where you are running the program from?
