# label not found in make.shared and collect.single
**URL:** https://forum.mothur.org/t/label-not-found-in-make-shared-and-collect-single/1627
**Category:** Commands in mothur
**Created:** [December 26, 2013, 8:22am UTC](https://forum.mothur.org/t/label-not-found-in-make-shared-and-collect-single/1627 "2013-12-26T08:22:21Z")
**Posts on this page:** 4
**Page:** 1
### Author:  [@elagon](https://forum.mothur.org/u/elagon)
#### Post date: [December 26, 2013, 8:22am UTC](https://forum.mothur.org/t/label-not-found-in-make-shared-and-collect-single/1627/1 "2013-12-26T08:22:21Z")
Hi, guys.
I’m using Mothur for microbiological analysis with PGM data. The tool is really awesome, and the SOP of Miseq helped a lot.
I used Mothur for several PGM project, and it turned out to be fine. But lately when I was analysing with “make.shared” command:
mothur \> make.shared(list=abc.trim.unique.good.filter.unique.precluster.pick.an.list, group=abc.group, label=0.03)
The output just print “0.02” and resulting files contain no information of distance 0.03.
Afterwards, I tried to calculate the shannon index of distance 0.03, with command
mothur \> collect.single(list=abc.trim.unique.good.filter.unique.precluster.pick.an.list, calc=shannon, label=0.03)
The output says “Your file does not include the label 0.03. I will use 0.02.”. Also no 0.03.
What the result was supposed to mean? Why was there no label of 0.03?
P.S. list file was generated by commands below:
mothur \> dist.seqs(fasta=abc.trim.unique.good.filter.unique.precluster.pick.fasta, cutoff=0.10)
mothur \> cluster(column=abc.trim.unique.good.filter.unique.precluster.pick.dist, name=abc.trim.unique.good.filter.unique.precluster.names, method=average)
Thanks in advance.
TYF
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### Author:  [@elagon](https://forum.mothur.org/u/elagon)
#### Post date: [January 2, 2014, 2:27am UTC](https://forum.mothur.org/t/label-not-found-in-make-shared-and-collect-single/1627/2 "2014-01-02T02:27:49Z")
Anyone who can help?
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### Author:  [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [January 2, 2014, 5:48pm UTC](https://forum.mothur.org/t/label-not-found-in-make-shared-and-collect-single/1627/3 "2014-01-02T17:48:35Z")
Here are some links you may find helpful:
[http://www.mothur.org/wiki/Frequently\_asked\_questions#Why\_does\_the\_cutoff\_change\_when\_I\_cluster\_with\_average\_neighbor.3F](http://www.mothur.org/wiki/Frequently_asked_questions#Why_does_the_cutoff_change_when_I_cluster_with_average_neighbor.3F)
[http://www.mothur.org/wiki/Frequently\_asked\_questions#Why\_is\_data\_missing\_for\_some\_distance\_levels.3F](http://www.mothur.org/wiki/Frequently_asked_questions#Why_is_data_missing_for_some_distance_levels.3F)
It looks like the cluster command adjusted your cutoff to 0.02, and that’s why the list file does not contain 0.03. You might try increasing your cutoff.
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### Author:  [@elagon](https://forum.mothur.org/u/elagon)
#### Post date: [January 3, 2014, 8:18am UTC](https://forum.mothur.org/t/label-not-found-in-make-shared-and-collect-single/1627/4 "2014-01-03T08:18:41Z")
> [@westcott](#):
>
> Here are some links you may find helpful:
>
> [Redirecting…](http://www.mothur.org/wiki/Frequently_asked_questions#Why_does_the_cutoff_change_when_I_cluster_with_average_neighbor.3F)
> [Redirecting…](http://www.mothur.org/wiki/Frequently_asked_questions#Why_is_data_missing_for_some_distance_levels.3F)
>
> It looks like the cluster command adjusted your cutoff to 0.02, and that’s why the list file does not contain 0.03. You might try increasing your cutoff.
westcott, thanks a lot.
I should have read FAQ first, such newbie questions.