# Killed: 9 while running dist.seqs **URL:** \ **Category:** mothur bugs\ **Created:** [February 7, 2019, 6:03am UTC](https://forum.mothur.org/t/killed-9-while-running-dist-seqs/19727 "2019-02-07T06:03:05Z")\ **Posts on this page:** 5\ **Page:** 1
**Author:** ![marclliros](https://avatars.discourse-cdn.com/v4/letter/m/82dd89/32.png) [@marclliros](https://forum.mothur.org/u/marclliros)\ **Post date:** [February 7, 2019, 6:03am UTC](https://forum.mothur.org/t/killed-9-while-running-dist-seqs/19727/1 "2019-02-07T06:03:05Z")
Dear all, I am running mothur v.1.41.3 on a macbook pro mid2014, 16Gb RAM and 500 Gb hard-drive (146Gb free). Files (except logfile) are stored in an external hard-drive of 2Tb (1 Tb free actually). I was following MiSeq SOP to analysePE 16S rRNA data from 25 samples generated using bacterial primers 341f-806br. All went well until dist.seqs that after a while stopped with message Killed: 9 I was wondering if it is a disk-space issue or RAM issue. My string of commands are as SOP but with … mothur \> dist.seqs(fasta=/Volumes/UAB\_data/0\_retosMassaMare\_reads/16S/0mm16Sfial.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.pick.fasta,cutoff=0.03) Using 8 processors. Sequence Time Num\_Dists\_Below\_Cutoff 0 0 0 100 0 439 200 0 2333 300 1 6581 400 1 12359 500 2 19543 600 2 29085 700 3 40485 800 4 54402 900 5 69233 1000 6 87956 1100 7 107772 1200 8 129343 1300 10 152164 1400 11 178614 151500 36 574722 … 61200 54268 296507129 289900 54305 246614293 222700 54325 300138472 163300 54336 291592655 269400 54346 250694100 61300 54338 297459110 247100 54377 313290006 123500 54407 257338194 195400 54431 235224760 61400 54417 298379120 61500 54491 299147360 163400 56932 294298768 123600 57051 259237683 Killed: 9 MBP-mlliros2:~ marclliros2$ after nearly 24h running Please could you give me your feedback? cheers Marc ---
**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [February 7, 2019, 6:28pm UTC](https://forum.mothur.org/t/killed-9-while-running-dist-seqs/19727/2 "2019-02-07T18:28:25Z")
Hi - I suspect it died because of disc space. Did you look to see how much was left as it was running? Pat ---
**Author:** ![marclliros](https://avatars.discourse-cdn.com/v4/letter/m/82dd89/32.png) [@marclliros](https://forum.mothur.org/u/marclliros)\ **Post date:** [February 7, 2019, 9:13pm UTC](https://forum.mothur.org/t/killed-9-while-running-dist-seqs/19727/3 "2019-02-07T21:13:05Z")
Hi Pat Thanks for your reply, I was looking by time to time, dist.seqs take time (it crashed after around 24-36h run), and as far as I remember it looks like there were still free space. However, please Pat correct me if I am wrong, but resulting file from dist.seqs (whatever.dist) should be “heavy”, right? Even crashed, there’s a file “whatever.dist” which is 220,23 Gb on disk. So I guess you’re right. I will try running it somewhere with more memory space. Really appreciate your comments Cheers Marc ---
**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\ **Post date:** [February 17, 2019, 9:17pm UTC](https://forum.mothur.org/t/killed-9-while-running-dist-seqs/19727/4 "2019-02-17T21:17:57Z")
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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [February 19, 2019, 4:11pm UTC](https://forum.mothur.org/t/killed-9-while-running-dist-seqs/19727/5 "2019-02-19T16:11:30Z")
Hmmm, I’m not really sure what’s happening. Regardless, even if you get dist.seqs to complete, there’s no way you’ll ever be able to cluster that distance matrix. I’d encourage you to check out cluster.split and this blog post on why people get such large distances matrices: [http://blog.mothur.org/2014/09/11/Why-such-a-large-distance-matrix/](http://blog.mothur.org/2014/09/11/Why-such-a-large-distance-matrix/)