I am running data from 58 samples through the mothur pipeline. Everything seems to be going fine until the cluster.split step. Every time I’ve left it to run, I come back and my computer has shut off (despite telling it not to do this while plugged in, and I can see that there are many .list files, but the one that should be used in the make.shared step seems to be empty? Is this an issue with computing power/RAM so it’s shutting off before completion?
Everything seems to be running fine to this point (number of seqs are comparable to a colleague doing a similarly sized group. I’m stumped. Please help!