# Issue in ASV make.shared

**URL:** <https://forum.mothur.org/t/issue-in-asv-make-shared/21099>\
**Category:** mothur bugs\
**Created:** [August 27, 2021, 3:03am UTC](https://forum.mothur.org/t/issue-in-asv-make-shared/21099 "2021-08-27T03:03:43Z")\
**Posts on this page:** 3\
**Page:** 1

<div class="post-metadata">

**Author:** ![BIKIM](https://avatars.discourse-cdn.com/v4/letter/b/e5b9ba/32.png) [@BIKIM](https://forum.mothur.org/u/BIKIM)\
**Post date:** [August 27, 2021, 3:03am UTC](https://forum.mothur.org/t/issue-in-asv-make-shared/21099/1 "2021-08-27T03:03:43Z")

</div>

I performed ASV analysis as the pipeline discribed in Miseq SOP:

pre.cluster(fasta=test2.unique.good.filter.unique.fasta, name=test2.unique.good.filter.names, group=test2.good.groups, diffs=15)  
chimera.uchime(fasta=test2.unique.good.filter.unique.precluster.fasta, count=test2.unique.good.filter.unique.precluster.count\_table)  
remove.seqs(accnos=test2.unique.good.filter.unique.precluster.denovo.uchime.accnos, fasta=test2.unique.good.filter.unique.precluster.fasta, count=test2.unique.good.filter.unique.precluster.count\_table, dups=T)  
classify.seqs(fasta=test2.unique.good.filter.unique.precluster.pick.fasta, count=test2.unique.good.filter.unique.precluster.pick.count\_table, reference=/home/disk2/DATABAE/QIIME2/silva-138-99-seqs.fasta, taxonomy=/home/disk2/DATABAE/QIIME2/silva-138-99.tax, cutoff=80,processors=30)  
make.shared(count=test2.unique.good.filter.unique.precluster.pick.count\_table)  
classify.otu(list=test2.unique.good.filter.unique.precluster.pick.ASV.list,count=test2.unique.good.filter.unique.precluster.pick.count\_table,taxonomy=test2.unique.good.filter.unique.precluster.pick.silva\_138\_99.wang.taxonomy,label=ASV)  
I noticed that make.shared step output wrong \*shared file.  
For example, Otu09245 size in file RNA.unique.good.pcr.filter.unique.precluster.pick.ASV.ASV.cons.taxonomy is 36370, however, in RNA.unique.good.pcr.filter.unique.precluster.pick.ASV.shared is 1.

Anyone encounter this issue？

---

<div class="post-metadata">

**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [September 1, 2021, 5:12pm UTC](https://forum.mothur.org/t/issue-in-asv-make-shared/21099/2 "2021-09-01T17:12:26Z")

</div>

It looks like the \*.ASV.shared file may not include the redundant sequences in the counts. We have made several improvements to the ASV workflow in our latest release, [Releases · mothur/mothur · GitHub](https://github.com/mothur/mothur/releases). Can you upgrade and if the problem persists send your input files and logfile to [mothur.bugs@gmail.com](mailto:mothur.bugs@gmail.com)?

---

<div class="post-metadata">

**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [September 11, 2021, 5:12pm UTC](https://forum.mothur.org/t/issue-in-asv-make-shared/21099/3 "2021-09-11T17:12:57Z")

</div>

This topic was automatically closed 10 days after the last reply. New replies are no longer allowed.