# How to get.oturep() when I don't have dist

**URL:** <https://forum.mothur.org/t/how-to-get-oturep-when-i-dont-have-dist/2492>\
**Category:** Commands in mothur\
**Created:** [August 11, 2015, 9:11pm UTC](https://forum.mothur.org/t/how-to-get-oturep-when-i-dont-have-dist/2492 "2015-08-11T21:11:14Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![yingeddi2008](https://avatars.discourse-cdn.com/v4/letter/y/e19adc/32.png) [@yingeddi2008](https://forum.mothur.org/u/yingeddi2008)\
**Post date:** [August 11, 2015, 9:11pm UTC](https://forum.mothur.org/t/how-to-get-oturep-when-i-dont-have-dist/2492/1 "2015-08-11T21:11:14Z")

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Hi All,

I am running OTU analysis based on MiSeq data. Since the final unique reads are so big: 176199 total reads, I used cluster.split() to bin them into OTU, so I ended up with 22441 OTUs. I want to perform UniFrac analysis based on OTU count table and OTU phylogenetic tree, so I will have to generate a phylogenetic tree from the representative OTU reads. And I was glad to find out that get.oturep() is available for me to extract the representative OTU. However, I am stuck at this step too, because the cluster.split() command didn’t produce a distance matrix to feed into the get.oturep command. All I have is a list file.

Could you please provide a way/command to extract representative OTU from what I have now?

I have a list file, OTU level shared file, final unique fasta file, and count\_table file.

Many thanks,

Eddi

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [August 13, 2015, 1:36pm UTC](https://forum.mothur.org/t/how-to-get-oturep-when-i-dont-have-dist/2492/2 "2015-08-13T13:36:22Z")

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Try using method=abundance in get.oturep.

Pat