# How can i start with mothur pipeline

**URL:** <https://forum.mothur.org/t/how-can-i-start-with-mothur-pipeline/20950>\
**Category:** Uncategorized\
**Created:** [April 6, 2021, 4:36pm UTC](https://forum.mothur.org/t/how-can-i-start-with-mothur-pipeline/20950 "2021-04-06T16:36:52Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![SAMRENDRA01](https://avatars.discourse-cdn.com/v4/letter/s/dc4da7/32.png) [@SAMRENDRA01](https://forum.mothur.org/u/SAMRENDRA01)\
**Post date:** [April 6, 2021, 4:36pm UTC](https://forum.mothur.org/t/how-can-i-start-with-mothur-pipeline/20950/1 "2021-04-06T16:36:52Z")

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Dear all the members, as i am new on the mothur, i do not understand how can i do the analysis of my 10 amplicon sequences with this pipeline can someone help me?

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [April 8, 2021, 5:53pm UTC](https://forum.mothur.org/t/how-can-i-start-with-mothur-pipeline/20950/2 "2021-04-08T17:53:58Z")

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Hi! Are you doing Sanger sequencing of 10 amplicons or something like MiSeq of 10 samples? If you are doing MiSeq, I’d encourage you to check out [MiSeq SOP](https://mothur.org/wiki/miseq_sop/). Alternatively, if you only have 10 sequences and all you need is their classification, I’d encourage you to use the online tools at [http://rdp.cme.msu.edu](http://rdp.cme.msu.edu)

Pat