# get.seqs from list file

**URL:** https://forum.mothur.org/t/get-seqs-from-list-file/147
**Category:** mothur bugs
**Created:** [February 12, 2010, 3:10pm UTC](https://forum.mothur.org/t/get-seqs-from-list-file/147 "2010-02-12T15:10:26Z")
**Posts on this page:** 4
**Page:** 1

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### Author: ![mbakker](https://avatars.discourse-cdn.com/v4/letter/m/82dd89/32.png) [@mbakker](https://forum.mothur.org/u/mbakker)
#### Post date: [February 12, 2010, 3:10pm UTC](https://forum.mothur.org/t/get-seqs-from-list-file/147/1 "2010-02-12T15:10:26Z")

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get.seqs doesn’t seem to be working correctly with list files.

For example:  
mothur \> list.seqs(group=AGSS.group)  
mothur \> get.seqs(accnos=AGSS.accnos, list=Ag\_Ss.list)  
mothur \> read.otu(list=Ag\_Ss.pick.list, label=0.01-0.03-0.05-0.10, group=AGSS.group)  
Your group file contains 194035 sequences and list file contains 194204 sequences. Please correct.  
For a list of names that are in your list file and not in your group file, please refer to Ag\_Ss.pick.missing.group.

Ag\_Ss.pick.missing.group contains 169 blank lines (no text at all).

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### Author: ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [February 23, 2010, 10:16am UTC](https://forum.mothur.org/t/get-seqs-from-list-file/147/2 "2010-02-23T10:16:01Z")

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Do you have duplicate names in your groupfile? If you want me to take a look you could send your files to [mothur.bugs@gmail.com](mailto:mothur.bugs@gmail.com).

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### Author: ![mbakker](https://avatars.discourse-cdn.com/v4/letter/m/82dd89/32.png) [@mbakker](https://forum.mothur.org/u/mbakker)
#### Post date: [March 5, 2010, 10:23pm UTC](https://forum.mothur.org/t/get-seqs-from-list-file/147/3 "2010-03-05T22:23:33Z")

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This seems to be a problem with extra commas left behind/carried forward in the list file.  
It works fine after I do a replace all ,\t with \t in a text editor.

This what the list file looks like with extra commas:  
…  
0.03 1238 Ag283a08\_196\_1\_1, Lc064a32\_3…  
0.04 925 Ss064a32\_575\_1\_1, Lp012a…  
…

And after removing them:  
…  
0.03 1238 Ag283a08\_196\_1\_1 Lc064a32\_3…  
0.04 925 Ss064a32\_575\_1\_1 Lp012a…  
…

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### Author: ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [March 10, 2010, 11:38am UTC](https://forum.mothur.org/t/get-seqs-from-list-file/147/4 "2010-03-10T11:38:04Z")

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Thanks for helping us find this bug. It will be fixed in 1.9
