# filter.seqs error

**URL:** <https://forum.mothur.org/t/filter-seqs-error/3093>\
**Category:** mothur bugs\
**Created:** [January 24, 2017, 7:13pm UTC](https://forum.mothur.org/t/filter-seqs-error/3093 "2017-01-24T19:13:02Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![tcolston6](https://avatars.discourse-cdn.com/v4/letter/t/aca169/32.png) [@tcolston6](https://forum.mothur.org/u/tcolston6)\
**Post date:** [January 24, 2017, 7:13pm UTC](https://forum.mothur.org/t/filter-seqs-error/3093/1 "2017-01-24T19:13:02Z")

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After running filter.seqs I’m finding that all of my columns have been removed. It doesn’t look like this forum has a search option anymore (at least there isn’t a search bar visible in my browser). I believe there’s already a thread for this but when I try to search this in google it won’t allow me access to those pages. All that comes up is the following error:

"Information

Sorry but you are not permitted to use the search system."

Any help with either issue is greatly appreciated!

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**Author:** ![dwaite](https://avatars.discourse-cdn.com/v4/letter/d/ecccb3/32.png) [@dwaite](https://forum.mothur.org/u/dwaite)\
**Post date:** [January 24, 2017, 10:07pm UTC](https://forum.mothur.org/t/filter-seqs-error/3093/2 "2017-01-24T22:07:14Z")

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I usually finds that this happens when the sequences haven’t been properly aligned/screened prior to filtering. What does the output of summary.seqs() on your aligned sequences look like?

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**Author:** ![tcolston6](https://avatars.discourse-cdn.com/v4/letter/t/aca169/32.png) [@tcolston6](https://forum.mothur.org/u/tcolston6)\
**Post date:** [January 25, 2017, 3:05pm UTC](https://forum.mothur.org/t/filter-seqs-error/3093/3 "2017-01-25T15:05:19Z")

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This is after alignseqs:

2.5%-tile: 1968 11550 252 0 4 270510  
25%-tile: 1968 11550 253 0 4 2705093  
Median: 1968 11550 253 0 5 5410186  
75%-tile: 1968 11550 253 0 6 8115278  
97.5%-tile: 1968 11550 253 0 6 10549861  
Maximum: 13425 13425 273 0 16 10820370  
Mean: 1972.54 11540.2 252.537 0 4.88631

# of unique seqs: 378237

total # of seqs: 10820370

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<div class="post-metadata">

**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [January 30, 2017, 1:45pm UTC](https://forum.mothur.org/t/filter-seqs-error/3093/4 "2017-01-30T13:45:48Z")

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In screen.seqs you need to say start=1968, end=11550. You have a sequence that starts and ends at 13425, so there is a . in every column. When you do filter.seqs(trump=T), that wipes out all of the columns.