# Fastq.info
**URL:** https://forum.mothur.org/t/fastq-info/778
**Category:** Commands in mothur
**Created:** [January 25, 2012, 4:51pm UTC](https://forum.mothur.org/t/fastq-info/778 "2012-01-25T16:51:40Z")
**Posts on this page:** 7
**Page:** 1
### Author:  [@antje0402](https://forum.mothur.org/u/antje0402)
#### Post date: [January 25, 2012, 4:51pm UTC](https://forum.mothur.org/t/fastq-info/778/1 "2012-01-25T16:51:40Z")
Hi Mothur developers and users,
I just started using Mothur for the analysis of SSU rRNA gene sequences generated on the Illumina platform.
The fastq.info command gives me the following error:
lengths do not match. read 150 characters for fasta and 69 characters for quality scores.
Sounds like there is a problem in reading out the quality information and I would appreciate any suggestions for solving that problem.
Thanx,
Antje
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### Author:  [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [January 25, 2012, 6:30pm UTC](https://forum.mothur.org/t/fastq-info/778/2 "2012-01-25T18:30:17Z")
Could you send your logfile and fastq file to [mothur.bugs@gmail.com](mailto:mothur.bugs@gmail.com)?
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### Author:  [@antje0402](https://forum.mothur.org/u/antje0402)
#### Post date: [January 26, 2012, 8:50pm UTC](https://forum.mothur.org/t/fastq-info/778/3 "2012-01-26T20:50:09Z")
Thanks!!
The fastq is quite large - but I sent the first lines - maybe it gets clear from that already what the problem might be. How long does it normally take for a reply?
[quote="westcott"] Could you send your logfile and fastq file to ? [/quote]
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### Author:  [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [January 27, 2012, 3:58pm UTC](https://forum.mothur.org/t/fastq-info/778/4 "2012-01-27T15:58:37Z")
The problem is occurring because the very last sequence in the file has an error. It looks like some of the quality information is missing. I will the sequence name to the error message output so in the future problems like this will be easier to spot.
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### Author:  [@antje0402](https://forum.mothur.org/u/antje0402)
#### Post date: [January 27, 2012, 4:02pm UTC](https://forum.mothur.org/t/fastq-info/778/5 "2012-01-27T16:02:09Z")
Thanks a lot!!
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### Author:  [@gwidmer](https://forum.mothur.org/u/gwidmer)
#### Post date: [August 22, 2012, 11:34pm UTC](https://forum.mothur.org/t/fastq-info/778/6 "2012-08-22T23:34:48Z")
Sarah/Pat, releated question: I used fastq.info to generate fasta files from illumina reads. the sequence names in fasta file look something like this: \>3VFXHS1:294:D17ETACXX:3:1101:17192:3058. This was apparently ok when running classify.seqs; but I attempted to run a UniFrac analysis pipeline with the same data and Unifrac.weighted crashed with the following message: -bash-3.2$ Name: 3VFXHS1 is not in your groupfile, and will be disregarded. The fact that the error message truncates the sequence name at the first “:” makes me think that “:” is not acceptable in sequence names. Is this so? I am sending the log file to mothur.bugs@gmail.
many thanks,
Giovanni
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### Author:  [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [August 24, 2012, 11:09am UTC](https://forum.mothur.org/t/fastq-info/778/7 "2012-08-24T11:09:48Z")
The problem is in the tree file. The ‘:’ character is a special character in a tree. It is used to indicate a branch length will follow.