# Fastq.info with only one read (Pacbio) **URL:** \ **Category:** mothur bugs\ **Created:** [August 6, 2019, 4:31pm UTC](https://forum.mothur.org/t/fastq-info-with-only-one-read-pacbio/20051 "2019-08-06T16:31:31Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![wolfgangrumpf](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/wolfgangrumpf/32/118_2.png) [@wolfgangrumpf](https://forum.mothur.org/u/wolfgangrumpf)\ **Post date:** [August 6, 2019, 4:31pm UTC](https://forum.mothur.org/t/fastq-info-with-only-one-read-pacbio/20051/1 "2019-08-06T16:31:31Z")
We’re using pacbio full-length 16S data in mothur to great success. However, when we do a multi-sample comparison, we have to construct all of the group files by hand. Fastq.info should be able to do this but it expects that the fastqFiles.file would have 3 columns: group, forward, and reverse read. With Pacbio there IS no forward/reverse read - the smartbell sequencing gives you a CSS (circular consensus). Is there a way that the next version of mothur could support single-read pacbio sequence in fastq.info (when the pacbio=T is set, of course)? ---
**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\ **Post date:** [August 16, 2019, 4:33pm UTC](https://forum.mothur.org/t/fastq-info-with-only-one-read-pacbio/20051/2 "2019-08-16T16:33:53Z")
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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\ **Post date:** [August 19, 2019, 4:04pm UTC](https://forum.mothur.org/t/fastq-info-with-only-one-read-pacbio/20051/3 "2019-08-19T16:04:59Z")
Thanks for the suggestion. Yes, we can add this to our next version. You can follow the progress here, [https://github.com/mothur/mothur/issues/649](https://github.com/mothur/mothur/issues/649).