# Error message

**URL:** https://forum.mothur.org/t/error-message/569
**Category:** Theory behind mothur
**Created:** [June 13, 2011, 5:28pm UTC](https://forum.mothur.org/t/error-message/569 "2011-06-13T17:28:45Z")
**Posts on this page:** 5
**Page:** 1

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### Author: ![fznajar](https://avatars.discourse-cdn.com/v4/letter/f/c57346/32.png) [@fznajar](https://forum.mothur.org/u/fznajar)
#### Post date: [June 13, 2011, 5:28pm UTC](https://forum.mothur.org/t/error-message/569/1 "2011-06-13T17:28:45Z")

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Hi,  
When running trim.seqs, I get the following message:

Appending files from process 45432  
[ERROR]: Could not open  
[ERROR]: LP2-Fares.fna45432.num.temp is blank. Please correct.

What does it mean?

Thanks a lot

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### Author: ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [June 17, 2011, 12:56pm UTC](https://forum.mothur.org/t/error-message/569/2 "2011-06-17T12:56:44Z")

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It looks like one of the processors encountered an error while running and quit. What version of mothur are you running?

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### Author: ![CDU](https://avatars.discourse-cdn.com/v4/letter/c/58956e/32.png) [@CDU](https://forum.mothur.org/u/CDU)
#### Post date: [September 18, 2012, 7:12am UTC](https://forum.mothur.org/t/error-message/569/3 "2012-09-18T07:12:13Z")

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I am getting a similar error message when I run align sequences ‘.unique.align23808.num.temp is blank. Please correct.’. I am running the latest version of mothur on a linux server system. Is this a way to correct this error?

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### Author: ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [September 18, 2012, 11:02am UTC](https://forum.mothur.org/t/error-message/569/4 "2012-09-18T11:02:54Z")

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Usually when one process dies it’s because of an error in the input file. Can you try running align.seqs with processors=1 to confirm?

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### Author: ![CDU](https://avatars.discourse-cdn.com/v4/letter/c/58956e/32.png) [@CDU](https://forum.mothur.org/u/CDU)
#### Post date: [September 18, 2012, 11:07pm UTC](https://forum.mothur.org/t/error-message/569/5 "2012-09-18T23:07:50Z")

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the alignment runs okay if I limit it to one processor. I found the problem - I didn’t have enough memory so the alignment files couldn’t be saved. Alignment size was 13gb and I only 9gb storage space free.
