# \[ERROR\] is in your groupfile and not your listfile
**URL:** https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2518
**Category:** Commands in mothur
**Created:** [September 1, 2015, 3:08pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2518 "2015-09-01T15:08:00Z")
**Posts on this page:** 4
**Page:** 1
### Author:  [@jcferc](https://forum.mothur.org/u/jcferc)
#### Post date: [September 1, 2015, 3:08pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2518/1 "2015-09-01T15:08:00Z")
hello everybody, I am Juan and currently having a “small” inconvenient
When i executed make.shared command to get OTU from each group, its present a following ERROR
make.shared(list=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.pick.an.unique\_list.list, count=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.denovo.uchime.pick.pick.count\_table, label=0.03)
[ERROR]: M01564\_51\_000000000-AG2K0\_1\_1102\_20256\_20354 is in your groupfile and not your listfile. Please correct.
Your group file contains 82403 sequences and list file contains 81279 sequences. Please correct.
Output File Names:
manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.pick.an.unique\_list.shared
* * *
Previously commands were
cluster.split(fasta=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.pick.fasta, count=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.denovo.uchime.pick.pick.count\_table, taxonomy=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.nr\_v119.wang.pick.taxonomy, splitmethod=classify, taxlevel=6, cutoff=0.15, processors=8)
Clustering manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.pick.fasta.7.dist
Cutoff was 0.155 changed cutoff to 0.07
Cutoff was 0.155 changed cutoff to 0.07
Cutoff was 0.155 changed cutoff to 0.07
It took 159 seconds to cluster
Merging the clustered files…
It took 15 seconds to merge.
Output File Names:
manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.pick.an.unique\_list.list
* * *
the count file was obtained with the following command
remove.lineage(fasta=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.fasta, count=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.denovo.uchime.pick.count\_table, taxonomy=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.nr\_v119.wang.taxonomy, taxon=Chloroplast-Mitochondria-unknown-Archaea-Eukaryota)
Output File Names:
manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.nr\_v119.wang.pick.taxonomy
manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.pick.pick.fasta
manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.denovo.uchime.pick.pick.count\_table
\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_
Sorry if I missed relevant info, is my first time using mothur and although I believe that it is very helpful, this OTU step is freaking me out
thank you very muc
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### Author:  [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [September 8, 2015, 6:06pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2518/2 "2015-09-08T18:06:55Z")
Could you post the uchime command and remove.seqs command you ran? I suspect you missed something there that is causing the file mismatch here.
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### Author:  [@jcferc](https://forum.mothur.org/u/jcferc)
#### Post date: [September 8, 2015, 6:11pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2518/3 "2015-09-08T18:11:05Z")
hello
thank you for your reply
sure! the commands are the following
chimera.uchime(fasta=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.fasta, count=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.count\_table, dereplicate=t)
remove.seqs(fasta=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.fasta, accnos=manglar.cut.trim.contigs.good.unique.good.filter.unique.precluster.abund.denovo.uchime.accnos)
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### Author:  [@westcott](https://forum.mothur.org/u/westcott)
#### Post date: [September 11, 2015, 3:34pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2518/4 "2015-09-11T15:34:10Z")
As a workaround you can run:
mothur \> list.seqs(list=yourListFile)
mothur \> get.seqs(count=yourCountFile)