# \[ERROR\] is in your groupfile and not your listfile.

**URL:** https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2340
**Category:** mothur bugs
**Created:** [April 2, 2015, 5:58pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2340 "2015-04-02T17:58:15Z")
**Posts on this page:** 1
**Page:** 1

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### Author: ![brooksph](https://avatars.discourse-cdn.com/v4/letter/b/67e7ee/32.png) [@brooksph](https://forum.mothur.org/u/brooksph)
#### Post date: [April 2, 2015, 5:58pm UTC](https://forum.mothur.org/t/error-is-in-your-groupfile-and-not-your-listfile/2340/1 "2015-04-02T17:58:15Z")

</div>

Hi,  
I keep running into this error and I have no idea what to do about it. I’ve tried 3 different versions of mothur (1.31, 1.33, and 1.34) with the same result.  
[ERROR]: HWI-M02808\_51\_A9K3P\_1\_1102\_6529\_19859 is in your groupfile and not your listfile. Please correct.  
[ERROR]: HWI-M02808\_51\_A9K3P\_1\_2107\_16825\_10620 is in your groupfile and not your listfile. Please correct.  
[ERROR]: HWI-M02808\_51\_A9K3P\_1\_2107\_17397\_16544 is in your groupfile and not your listfile. Please correct.  
[ERROR]: HWI-M02808\_51\_A9K3P\_1\_2112\_5166\_10436 is in your groupfile and not your listfile. Please correct.  
Your group file contains 136663 sequences and list file contains 136659 sequences. Please correct.

The error occurs when I run the make.shared command:  
make.shared(list=quality\_sequences.an.unique\_list.list, count=quality\_sequences.count\_table, label=0.03)

The commands that I ran prior to this step are as follows:

make.contigs(file=cecal\_transfer.files, processors=8)

screen.seqs(fasta=cecal\_transfer.trim.contigs.fasta, group=cecal\_transfer.contigs.groups, maxambig=0, maxlength=275)

unique.seqs(fasta=current)

count.seqs(name=current, group=current)

pcr.seqs(fasta=silva.bacteria.fasta, start=11894, end=25319, keepdots=F, processors=8)

system(mv silva.bacteria.pcr.fasta silva.v4.fasta)

align.seqs(fasta=cecal\_transfer.trim.contigs.good.unique.fasta, reference=silva.v4.fasta)

screen.seqs(fasta=current, count=current, summary=current, start=1968, end=11550, maxhomop=8)

filter.seqs(fasta=cecal\_transfer.trim.contigs.good.unique.good.align, vertical=T, trump=.)

unique.seqs(fasta=current, count=current)

pre.cluster(fasta=current, count=current, diffs=2)

chimera.uchime(fasta=current, count=current, dereplicate=t)

remove.seqs(fasta=current, accnos=current)

classify.seqs(fasta=current, count=current, reference=trainset9\_032012.pds.fasta, taxonomy=trainset9\_032012.pds.tax, cutoff=80)

remove.lineage(fasta=current, count=current, taxonomy=current, taxon=Chloroplast-Mitochondria-unknown-Archaea-Eukaryota)

cp cecal\_transfer.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.fasta quality\_sequences.fasta

cp cecal\_transfer.trim.contigs.good.unique.good.filter.unique.precluster.uchime.pick.pick.count\_table quality\_sequences.count\_table quality\_sequences.count\_table

cp cecal\_transfer.trim.contigs.good.unique.good.filter.unique.precluster.pick.pds.wang.pick.taxonomy quality\_sequences.taxonomy

cp cecal\_transfer.trim.contigs.good.unique.good.filter.unique.precluster.pick.pick.pick.an.unique\_list.list quality\_sequences.an.unique\_list.list

set.current(fasta=quality\_sequences.fasta, count=quality\_sequences.count\_table, taxonomy=quality\_sequences.taxonomy, processors=8)

dist.seqs(fasta=current, cutoff=0.2)

cluster.split(column=current, count=current, taxonomy=current, splitmethod=classify, taxlevel=4, cutoff=0.15)