# error for uchime and chimera slayer on linux system **URL:** \ **Category:** mothur bugs\ **Created:** [September 21, 2012, 1:32am UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052 "2012-09-21T01:32:27Z")\ **Posts on this page:** 7\ **Page:** 1
**Author:** ![CDU](https://avatars.discourse-cdn.com/v4/letter/c/58956e/32.png) [@CDU](https://forum.mothur.org/u/CDU)\ **Post date:** [September 21, 2012, 1:32am UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/1 "2012-09-21T01:32:27Z")
I am currently running mothur on a linux server system. When I use the uchime command I receive the following error message Using 1 processors. /usr/local/bin/uchime file does not exist. Checking path… [ERROR]: file does not exist. mothur requires the uchime executable. [ERROR]: did not complete chimera.uchime. I also get the following error messages when I try chimera slayer Only reporting sequence supported by 90% of bootstrapped results. [ERROR]: /usr/local/bin/blast/bin/formatdb file does not exist. mothur requires formatdb.exe. [ERROR]: /usr/local/bin/blast/bin/blastall file does not exist. mothur requires blastall.exe. [ERROR]: /usr/local/bin/blast/bin/megablast file does not exist. mothur requires megablast.exe. I have run the analysis on a stand alone desktop with the same files it works okay. Does anyone know how to fix the error on the linux system? thanks ---
**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\ **Post date:** [September 24, 2012, 11:10am UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/2 "2012-09-24T11:10:16Z")
For chimera.uchime, did you try moving the uchime executable to /usr/local/bin/uchime? For chimera.slayer, you can move the blast folder to /usr/local/bin, or set your blast location using the blastlocation parameter. ---
**Author:** ![HilaE](https://avatars.discourse-cdn.com/v4/letter/h/aca169/32.png) [@HilaE](https://forum.mothur.org/u/HilaE)\ **Post date:** [December 13, 2012, 12:09pm UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/3 "2012-12-13T12:09:49Z")
Hello We encountered the same problem. We tried to create a “local” library in our user name directory as suggested but with no success. It look like Mothur cannot find the UCHIME. Any suggestions? thank you Hila ---
**Author:** ![dwaite](https://avatars.discourse-cdn.com/v4/letter/d/ecccb3/32.png) [@dwaite](https://forum.mothur.org/u/dwaite)\ **Post date:** [December 13, 2012, 7:02pm UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/4 "2012-12-13T19:02:31Z")
I’ve never had this problem on our linux computers, but I have noticed that if you try to run mothur installed in the QIIME virtual machine, the same problem happens with uchime - it’s in the same directory as mothur, but can’t be found. ---
**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\ **Post date:** [December 14, 2012, 11:45am UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/5 "2012-12-14T11:45:48Z")
Have you tried putting uchime in the same folder as mothur’s exe? ---
**Author:** ![Tom42](https://avatars.discourse-cdn.com/v4/letter/t/ea666f/32.png) [@Tom42](https://forum.mothur.org/u/Tom42)\ **Post date:** [September 17, 2015, 11:40am UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/6 "2015-09-17T11:40:19Z")
i have encountered a similar problem. May i ask what is the uchime executable?? I have downloaded both “gold” database and uchine2.40 from their site but nothing worked.shall i change the name of the file or something ? ---
**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\ **Post date:** [September 17, 2015, 5:10pm UTC](https://forum.mothur.org/t/error-for-uchime-and-chimera-slayer-on-linux-system/1052/7 "2015-09-17T17:10:20Z")
The uchime executable is released with the executable versions of mothur, [https://github.com/mothur/mothur/releases/tag/v1.36.1](https://github.com/mothur/mothur/releases/tag/v1.36.1). It is also built as part the build process when you build mothur from source yourself. The file will be named uchime on Linux/Max machines, or uchime.exe on Windows machines. Mothur’s wrapper command, chimera.uchime [http://www.mothur.org/wiki/Chimera.uchime](http://www.mothur.org/wiki/Chimera.uchime), is design to work with the version of uchime released with mothur’s executable and source. Mothur’s chimera.uchime command prepares the inputs, runs the uchime executable, and assembles the results.