# Do pcr.seqs() allow IUPAC ambiguity codes?

**URL:** <https://forum.mothur.org/t/do-pcr-seqs-allow-iupac-ambiguity-codes/1564>\
**Category:** Commands in mothur\
**Created:** [October 31, 2013, 11:09am UTC](https://forum.mothur.org/t/do-pcr-seqs-allow-iupac-ambiguity-codes/1564 "2013-10-31T11:09:36Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![maasha](https://avatars.discourse-cdn.com/v4/letter/m/5fc32e/32.png) [@maasha](https://forum.mothur.org/u/maasha)\
**Post date:** [October 31, 2013, 11:09am UTC](https://forum.mothur.org/t/do-pcr-seqs-allow-iupac-ambiguity-codes/1564/1 "2013-10-31T11:09:36Z")

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Do pcr.seqs() allow IUPAC ambiguity codes in sequences and primers?

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [November 14, 2013, 9:03pm UTC](https://forum.mothur.org/t/do-pcr-seqs-allow-iupac-ambiguity-codes/1564/2 "2013-11-14T21:03:30Z")

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It should for the primers. Any degenerate bases will be converted to N’s in the sequences.