# Diffs value for pre.cluster

**URL:** <https://forum.mothur.org/t/diffs-value-for-pre-cluster/20571>\
**Category:** Theory behind mothur\
**Created:** [June 15, 2020, 9:41pm UTC](https://forum.mothur.org/t/diffs-value-for-pre-cluster/20571 "2020-06-15T21:41:34Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![DEEPCHANDA7](https://avatars.discourse-cdn.com/v4/letter/d/eb9ed0/32.png) [@DEEPCHANDA7](https://forum.mothur.org/u/DEEPCHANDA7)\
**Post date:** [June 15, 2020, 9:41pm UTC](https://forum.mothur.org/t/diffs-value-for-pre-cluster/20571/1 "2020-06-15T21:41:34Z")

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Hi there!!! @pschloss @westcott  
Can I take diffs=2 value for the pre.cluster step for read length 450 bp?

Thanks and regards,  
DC7

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [June 16, 2020, 1:00am UTC](https://forum.mothur.org/t/diffs-value-for-pre-cluster/20571/2 "2020-06-16T01:00:51Z")

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I’d encourage you to read

Schloss PD, Gevers D, Westcott SL (2011). Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. PLoS ONE. 6:e27310.

It gives a rationale for the number of diffs to use.

Pat

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**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [June 26, 2020, 1:00am UTC](https://forum.mothur.org/t/diffs-value-for-pre-cluster/20571/3 "2020-06-26T01:00:56Z")

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