difference between sabund and rabund outputs

Commands in mothur
#1

sabund output:
unique 4 7682 2 0 1
0.01 4 5118 1249 22 2
0.02 127 1881 609 255 111 65 20
0.03 192 1505 430 188 81 61 26
0.04 299 1158 378 139 82 55 28
0.05 317 1039 355 137 80 54 34

end of sabund file
0.63 7501 2 2 1 0
0.64 7502 1 2 1 0
0.66 7502 1 2 1 0
0.67 7521 1 2 1 0
0.7 7568 1 2 1 0
0.71 7568 0 2 1 0
0.74 7675 0 1 0 0
0.86 7675 0 1 0 0
1 7690 0 0 0 0
rabund output:
unique 7685 4 2 2 1 1
0.01 6391 4 4 3 3 3
0.02 3070 127 115 114 110 90
0.03 2446 192 179 170 160 156
0.04 2007 299 207 204 165 164
0.05 1864 317 221 206 178 171

Hello Everyone,
I will be grateful if anyone can explain be the difference between these two outputs.
On manual it says that in sabund the second column is number of sequences in the dominant OTU(s) and in rabund the second column is the number of OTUs that have been formed.
In the sabund file why the second column is increasing ?


Thanks!!!!
#2

The sabund data reported in an sabund file is appropriate for plotting in a species-abundance plot, http://www.mothur.org/wiki/Sabund_file.

0.03 192 1505 430 188 81 61 26

In the above example, 192 is the number of OTUs at distance 0.03. 1505 is the number of OTUs containing 1 sequence , 430 is the the number of OTUs containing 2 sequences, 188 is the number of OTU’s containing 3 sequences, ect.

The data reported in the rabund file is appropriate for plotting as a rank-abundance plot, http://www.mothur.org/wiki/Rabund_file.

0.03 2446 192 179 170 160 156

In the above example, 2446 is the number of OTUs at distance 0.03. 192 is the number of sequences in OTU1 , 179 is the number of sequences in OTU2, 170 is the number of sequences in OTU3, ect.

I hope this helps,
Sarah

#3

Hi Sarah,
Thanks for the help!!

"0.03 192 1505 430 188 81 61 26

In the above example, 192 is the number of OTUs at distance 0.03. 1505 is the number of OTUs containing 1 sequence , 430 is the the number of OTUs containing 2 sequences, 188 is the number of OTU’s containing 3 sequences, ect."

I didn’t get this one. If the number of OTUs are 192 at distance 0.03 then how come 1505 is the number of OTUs containing sequence 1,430 is the the number of OTUs containing 2 sequences…etc…
will not all the OTUs sum to 192?
Thanks!!!

#4

Sorry for the confusion. In the above example, 192 is the number of sequences in the dominant or largest OTU for the sabund output.