# Classify.seqs

**URL:** <https://forum.mothur.org/t/classify-seqs/2237>\
**Category:** Commands in mothur\
**Created:** [February 5, 2015, 2:43pm UTC](https://forum.mothur.org/t/classify-seqs/2237 "2015-02-05T14:43:21Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![anpoosak](https://avatars.discourse-cdn.com/v4/letter/a/85e7bf/32.png) [@anpoosak](https://forum.mothur.org/u/anpoosak)\
**Post date:** [February 5, 2015, 2:43pm UTC](https://forum.mothur.org/t/classify-seqs/2237/1 "2015-02-05T14:43:21Z")

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Hi,  
Is it possible to use .shared file for classify.seqs?

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [February 5, 2015, 5:55pm UTC](https://forum.mothur.org/t/classify-seqs/2237/2 "2015-02-05T17:55:15Z")

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No, but perhaps you are looking for the classify.otu command, [http://www.mothur.org/wiki/Classify.otu](http://www.mothur.org/wiki/Classify.otu). It requires a list file.

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**Author:** ![anpoosak](https://avatars.discourse-cdn.com/v4/letter/a/85e7bf/32.png) [@anpoosak](https://forum.mothur.org/u/anpoosak)\
**Post date:** [February 5, 2015, 6:04pm UTC](https://forum.mothur.org/t/classify-seqs/2237/3 "2015-02-05T18:04:29Z")

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Thanks for your reply. I am trying to make sub samples of my data to normalise them and use them for further classification! I used make.shar for sub sampling, but i have no idea how to proceed further!

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**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\
**Post date:** [February 5, 2015, 8:45pm UTC](https://forum.mothur.org/t/classify-seqs/2237/4 "2015-02-05T20:45:26Z")

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You would want to subsample the list, taxonomy, names and group files. Then run the make.shared command.

mothur \> sub.sample(list=yourListFile, taxonomy=yourTaxonomyfile, name=yourNameFile, group=yourGroupFile, persample=t)  
mothur \> make.shared(list=current, group=current) - create a shared file from your subsampled dataset  
mothur \> classify.otu(list=current, group=current, taxonomy=current, name=current) - classify subsampled otus.

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**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [February 6, 2015, 12:51pm UTC](https://forum.mothur.org/t/classify-seqs/2237/5 "2015-02-06T12:51:09Z")

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But really, there is no reason to subsample the taxonomy data since the consensus taxonomy won’t change