chimera.uchime "bug"?

Okay, it’s entirely possible this is intentional and not a bug. But it shut down one of my mothur runs and gave me fits trying to figure out what the problem was so here it is:

chimera.uchime is apparently set up to accept multiple fasta filenames following the fasta= tag, with each filename separated by a hyphen. This is similar to the make.groups command, for instance. However, in the unusual instance where a filename actually INCLUDES a hyphen, chimera.uchime blows up. Here’s the filename I tried to check that shut me down:

CAM_PROJ_WesternChannelOMM.read.WESTERNCHANNELOMM_SMPL_154-APR_NIGHT_PREFILTER.fasta

So, this isn’t exactly a bug – but it might be worth either removing the multiple file option or mentioning in the wiki that hyphens are a no-no.

You should be able to put a \ in front of the dash and get it to work. Although not including dashes in group/file names is a good idea :slight_smile: