# chimera.uchime and merge.files commands **URL:** \ **Category:** Commands in mothur\ **Created:** [July 21, 2014, 12:51pm UTC](https://forum.mothur.org/t/chimera-uchime-and-merge-files-commands/1930 "2014-07-21T12:51:45Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![Mariana\_Reis](https://avatars.discourse-cdn.com/v4/letter/m/ebca7d/32.png) [@Mariana\_Reis](https://forum.mothur.org/u/Mariana_Reis)\ **Post date:** [July 21, 2014, 12:51pm UTC](https://forum.mothur.org/t/chimera-uchime-and-merge-files-commands/1930/1 "2014-07-21T12:51:45Z")
Hello! I’m having 2 different problems with 2 analyses: In the first analysis, I can’t complete the chimera.uhime command. The accnos file was not complete and the error message below was exhibited: [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_7359\_15838 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_14555\_15870 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_27464\_16008 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_20966\_16078 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_22987\_16117 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_15596\_16123 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_26544\_16416 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_4317\_16619 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_12838\_16623 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_23959\_16653 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_16320\_17060 with a total=0. Please correct. [ERROR]: Your count table contains a sequence named M01967\_11\_000000000-A7L66\_1\_2113\_9803\_17066 with a total=0. Please correct. What can I do? The second problem is in other file and other analysis. I have to merge files in order to comparing two microbiomes. So, I merged the taxonomy, count\_table and fasta files from both microbiomes, which were generated after the remove.lineage command. However, when I performed the next step which was the cluster.split command, it was started, but in few minutes was interrupted. The error message said that the fasta file used is not unique… Could someone help me? Thanks a lot!!! ---
**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\ **Post date:** [July 24, 2014, 3:59pm UTC](https://forum.mothur.org/t/chimera-uchime-and-merge-files-commands/1930/2 "2014-07-24T15:59:34Z")
> The second problem is in other file and other analysis. I have to merge files in order to comparing two microbiomes. So, I merged the taxonomy, count\_table and fasta files from both microbiomes, which were generated after the remove.lineage command. However, when I performed the next step which was the cluster.split command, it was started, but in few minutes was interrupted. The error message said that the fasta file used is not unique… You can’t merge the count\_tables. Can you try merging your data before running count.seqs and see if both problems go away? pat