Blank file after screen

After screen, i have blank file.

I tell you the reference.
When i use iseq machine, i registered read lengths.
Read 1 is 150 and Read 2 is 150.
Is it problem?
And tell me the resolve the problem.

I will wait your response of it.
Thank you.

Hi - can you post the output of running summary.seqs on the file you gave to screen.seqs?

Also, 2x150 should work, but know that if the region you are sequencing is longer than 150 nt long, you will get higher error rates than we do with fully overlapping reads. See this blog post…