# .biom table for PICRUSt in Galaxy returning errors **URL:** \ **Category:** Integrating mothur with other programs\ **Created:** [August 24, 2016, 7:15pm UTC](https://forum.mothur.org/t/biom-table-for-picrust-in-galaxy-returning-errors/2970 "2016-08-24T19:15:44Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![met615](https://avatars.discourse-cdn.com/v4/letter/m/a4c791/32.png) [@met615](https://forum.mothur.org/u/met615)\ **Post date:** [August 24, 2016, 7:15pm UTC](https://forum.mothur.org/t/biom-table-for-picrust-in-galaxy-returning-errors/2970/1 "2016-08-24T19:15:44Z")
Hi all, I’m not sure this is the best place to ask this, but I figured it was worth a shot. I generated a .biom table using the .cons.taxonomy I generated in mothur. I used the GreenGenes version specified by Galaxy (13.5) and I removed the unknown taxa using the remove.lineages command during this process. I input this file into the Galaxy online version to run PICRUSt. It generates this error when I try to use normalize by copy number: ValueError: No OTUs match identifiers in precalculated file. PICRUSt requires an OTU table reference/closed picked against GreenGenes. Example of the first 5 OTU ids from your table: Otu02710, Otu02711, Otu02712, Otu02713, Otu02714 I don’t understand why this error comes up or why it can get through 2709 OTUs before stopping. Any thoughts/ideas/suggestions would be super helpful and much appreciated! I tried getting help from the lab that runs Galaxy but haven’t received a response. Thanks! Morgan ---
**Author:** ![westcott](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/westcott/32/18_2.png) [@westcott](https://forum.mothur.org/u/westcott)\ **Post date:** [August 31, 2016, 1:51pm UTC](https://forum.mothur.org/t/biom-table-for-picrust-in-galaxy-returning-errors/2970/2 "2016-08-31T13:51:18Z")
Could you post the command you ran and a sample of your biom file?