# any idea why all sequences are shown in a separate OTU?

**URL:** <https://forum.mothur.org/t/any-idea-why-all-sequences-are-shown-in-a-separate-otu/1997>\
**Category:** Commands in mothur\
**Created:** [August 29, 2014, 6:33pm UTC](https://forum.mothur.org/t/any-idea-why-all-sequences-are-shown-in-a-separate-otu/1997 "2014-08-29T18:33:35Z")\
**Posts on this page:** 3\
**Page:** 1

<div class="post-metadata">

**Author:** ![pavel47](https://avatars.discourse-cdn.com/v4/letter/p/a698b9/32.png) [@pavel47](https://forum.mothur.org/u/pavel47)\
**Post date:** [August 29, 2014, 6:33pm UTC](https://forum.mothur.org/t/any-idea-why-all-sequences-are-shown-in-a-separate-otu/1997/1 "2014-08-29T18:33:35Z")

</div>

Hello there,

I’m trying to follow the 454 SOP and I have run into a problem when classifying into OTUs. I have a fasta, names and group file and am trying to run ‘dist.seqs’ and then ‘cluster’ on the resulting dist file. When I do this all 18000 sequences are classified into a separate OTU, (giving me 18000 OTUs) and the only cutoff shown is ‘unique’. Does anyone have any tips or ideas about what could be going on?

Thanks  
Pavel

---

<div class="post-metadata">

**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [August 30, 2014, 12:51pm UTC](https://forum.mothur.org/t/any-idea-why-all-sequences-are-shown-in-a-separate-otu/1997/2 "2014-08-30T12:51:36Z")

</div>

Check out:

[http://www.mothur.org/wiki/Frequently\_asked\_questions#Why\_is\_my\_dataset\_only\_clustering\_to\_.22unique.22.3F](http://www.mothur.org/wiki/Frequently_asked_questions#Why_is_my_dataset_only_clustering_to_.22unique.22.3F)  
[http://www.mothur.org/wiki/Frequently\_asked\_questions#Why\_does\_the\_cutoff\_change\_when\_I\_cluster\_with\_average\_neighbor.3F](http://www.mothur.org/wiki/Frequently_asked_questions#Why_does_the_cutoff_change_when_I_cluster_with_average_neighbor.3F)

Pat

---

<div class="post-metadata">

**Author:** ![pavel47](https://avatars.discourse-cdn.com/v4/letter/p/a698b9/32.png) [@pavel47](https://forum.mothur.org/u/pavel47)\
**Post date:** [September 3, 2014, 12:01am UTC](https://forum.mothur.org/t/any-idea-why-all-sequences-are-shown-in-a-separate-otu/1997/3 "2014-09-03T00:01:58Z")

</div>

Ah ok, thanks.