# Add you feature for subsample.seqs command

**URL:** <https://forum.mothur.org/t/add-you-feature-for-subsample-seqs-command/21615>\
**Category:** Feature requests\
**Created:** [November 9, 2022, 6:35am UTC](https://forum.mothur.org/t/add-you-feature-for-subsample-seqs-command/21615 "2022-11-09T06:35:08Z")\
**Posts on this page:** 3\
**Page:** 1

<div class="post-metadata">

**Author:** ![josef](https://avatars.discourse-cdn.com/v4/letter/j/ec9cab/32.png) [@josef](https://forum.mothur.org/u/josef)\
**Post date:** [November 9, 2022, 6:35am UTC](https://forum.mothur.org/t/add-you-feature-for-subsample-seqs-command/21615/1 "2022-11-09T06:35:08Z")

</div>

Dear Patrick,

I am always using Mother with great joy since the introduction of Dothur.  
I would like to suggest to add the following extra feature to the subsample.seq command.  
I usually supsample to somewhat around 50,000 reads per samples and afterwards I add any other samples back between 10,000 and 50,000 reads per samples. This always involves some additional steps.  
So, it would be fantastic if you could add a parameter to the subsample command which allows to subsample to a range of numbers. Example I would like 50,000 reads per sample and also leave all other samples in, which as a user defined smaller number e.g., 10,000 reads. That would be really cool. Thank you, Josef

---

<div class="post-metadata">

**Author:** ![system](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/system/32/2_2.png) [@system](https://forum.mothur.org/u/system)\
**Post date:** [November 19, 2022, 6:35am UTC](https://forum.mothur.org/t/add-you-feature-for-subsample-seqs-command/21615/2 "2022-11-19T06:35:46Z")

</div>

This topic was automatically closed 10 days after the last reply. New replies are no longer allowed.

---

<div class="post-metadata">

**Author:** ![pschloss](https://yyz2.discourse-cdn.com/flex036/user_avatar/forum.mothur.org/pschloss/32/4_2.png) [@pschloss](https://forum.mothur.org/u/pschloss)\
**Post date:** [November 21, 2022, 3:48pm UTC](https://forum.mothur.org/t/add-you-feature-for-subsample-seqs-command/21615/3 "2022-11-21T15:48:08Z")

</div>

Hi Josef,

Thanks for your post. I’d suggest running summary.single twice at multiple read depths and changing the output file name after each run.

Pat